It's not currently possible to split family trees on Ancestry®, but there are two ways to get similar results: duplicating your tree, and saving people from one tree to another.
Duplicating your tree
To create a duplicate tree on Ancestry®, download the tree you want to duplicate, then upload it again as a separate tree. After creating a duplicate tree, you can delete the people that you don't want to include in the new tree.
When you download a tree from Ancestry, it comes in the form of a GEDCOM file. GEDCOM files are text-only, which means that any media attached to your original tree won't be included in the second tree. Media items will need to be added to the duplicate tree from the original. Facts, notes, and sources are usually retained by a file in GEDCOM format after conversion.
If you plan to change both trees after creating the duplicate tree, we recommend keeping your original tree as a backup and downloading two copies.
Saving people to your tree
People can be copied either from public family trees posted by other members or from other trees on your own account. Copying people from other trees on your account can be useful when trying to move people between trees.
You can save people from one tree to another on a person-by-person basis (meaning that only one person may be copied at a time).